If a probe is made of radioactively labelled dsDNA and is allowed to hybridise to its complementary DNA in a cloned cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?

Options

A. It is double stranded DNA
B. It is radioactively labelled
C. This probe can be used in autoradiography
D. It is hybridise to complementary DNA/RNA

Answer

To solve the question regarding the use of a radioactively labeled double-stranded DNA (dsDNA) probe and its hybridization with complementary DNA in a cloned cell, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Probe**:
- The question states that we have a probe made of radioactively labeled dsDNA. This means that the DNA probe has two strands and is labeled with a radioactive isotope for detection purposes.

2. **Hybridization Process**:
- Hybridization refers to the process where the probe binds to its complementary DNA or RNA sequence. For hybridization to occur effectively, the probe must be able to form hydrogen bonds with the complementary strands.

3. **Nature of the Probe**:
- It is important to note that probes used in hybridization are typically single-stranded. A single-stranded DNA probe can easily bind to a complementary strand by forming hydrogen bonds.

4. **Analyzing the Statements**:
- The first statement claims that the probe is double-stranded. This is correct as per the question.
- The second statement mentions that the probe is radioactively labeled, which is also correct.
- The third statement indicates that the probe can be used in autoradiography, which is true since the radioactive label allows for detection.
- The fourth statement claims that the probe hybridizes to complementary DNA and RNA. This is incorrect because a double-stranded probe cannot hybridize with complementary strands; it must be single-stranded to do so.

5. **Conclusion**:
- The statement that is considered wrong is the last one, which suggests that the double-stranded probe can hybridize with complementary DNA and RNA. This is not possible because a double-stranded probe cannot effectively hybridize.

### Final Answer:
The statement that is considered wrong is: "It is hybridized to complementary DNA and RNA."

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