The main challenge for production of insulin using rDNA techniques was getting insulin into matured form using _________ bond

Options

A. Hydrogen
B. Peptide
C. Ionic
D. Disulphide

Answer

To answer the question regarding the main challenge for the production of insulin using recombinant DNA (rDNA) techniques, we need to focus on the type of bond that is crucial for the maturation of insulin.

1. **Understanding Insulin Structure**: Insulin is a peptide hormone composed of two chains, known as the A chain and the B chain. These chains are initially synthesized separately in bacterial systems, such as E. coli, when using rDNA technology.

2. **Challenges in Production**: The primary challenge in producing functional insulin is that the A and B chains need to be linked together correctly to form the active hormone. When these chains are produced separately, they do not naturally come together to form the mature insulin.

3. **Formation of Mature Insulin**: For insulin to become biologically active, the A and B chains must be connected by specific types of bonds. In the case of insulin, these are disulfide bonds. Disulfide bonds are formed between the sulfur atoms of cysteine residues in the A and B chains.

4. **Final Answer**: Therefore, the main challenge for the production of insulin using rDNA techniques was getting insulin into its matured form using **disulfide bonds**.