You are given a tissue with its potential for differentiation in an artificial culture .Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots

Options

A. IAA and gibberellin
B. Auxin and cytokinin
C. Auxin and abscsic acid
D. Gibberellin and abscisic acid

Answer

To determine the appropriate pair of hormones to add to a tissue culture medium for securing both shoot and root development, follow these steps:

### Step-by-Step Solution:

1. **Understand the Role of Hormones in Plant Growth**:
- Plant hormones, or phytohormones, play a crucial role in regulating growth and development. The two main types of hormones involved in shoot and root formation are auxins and cytokinins.

2. **Identify the Functions of Auxins and Cytokinins**:
- **Auxins**: Promote cell elongation, root formation, and are crucial for the development of shoots.
- **Cytokinins**: Promote cell division and are essential for shoot formation.

3. **Consider Other Hormones**:
- **Abscisic Acid (ABA)**: Generally acts as a growth inhibitor and is not used for promoting shoot or root development.
- **Gibberellins**: Primarily promote stem elongation and seed germination, but do not significantly influence root formation.

4. **Combine the Right Hormones**:
- For effective tissue culture that encourages both shoot and root development, a combination of auxins and cytokinins is ideal. Auxins will help in root formation while cytokinins will support shoot development.

5. **Select the Correct Option**:
- Based on the analysis, the correct pair of hormones to add to the medium is **Auxin and Cytokinin**.

### Final Answer:
The correct pair of hormones to add to the medium to secure both shoots and roots is **Auxin and Cytokinin**.

---