The viability of seeds is tested by

Options

A. Safranin
B. 2,6 dichlorophenol indophenols
C. 2,3,5 triphenyl tetrazolium chloride
D. DMSO

Answer

To determine the viability of seeds, follow these steps:

### Step 1: Understand Seed Viability
- **Definition**: Seed viability refers to the ability of seeds to germinate and develop into a healthy plant. Seeds lose viability over time, and testing is necessary to determine if they can still germinate.

### Step 2: Identify the Testing Substance
- **Substance Used**: The viability of seeds can be tested using a chemical called triphenyl tetrazolium chloride (TTC). This compound is crucial for assessing the metabolic activity of the seed embryos.

### Step 3: Prepare the Solution
- **Concentration**: Prepare a 0.1% solution of triphenyl tetrazolium chloride. This concentration is effective for the viability test.

### Step 4: Immerse the Seeds
- **Testing Procedure**: Immerse a section of the seed in the prepared TTC solution. This allows the chemical to interact with the seed's embryo.

### Step 5: Observe Color Change
- **Observation**: After immersion, observe the solution. If the seeds are viable, the embryo will respire and produce carbon dioxide. This gas will react with the TTC, causing the colorless solution to turn pink.

### Step 6: Interpretation of Results
- **Conclusion**: The pink coloration indicates that the embryo is alive and respiring, confirming that the seeds are viable. If there is no color change, the seeds are likely non-viable.

### Final Answer
- The viability of seeds is tested by immersing them in a 0.1% solution of triphenyl tetrazolium chloride, which turns pink if the seeds are viable.

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