Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

Options

A. On plates with and without streptomycin
B. On plates with minimal medium
C. Only on plates with streptomycin
D. Only on plates without streptomycin.

Answer

**Step-by-Step Solution:**

1. **Understanding the Context**: The question involves selecting streptomycin-resistant mutants from bacterial colonies using imprints from a plate with complete medium. Streptomycin is an antibiotic that inhibits bacterial growth.

2. **Identifying the Types of Media**: There are two types of media to consider:
- **Complete Medium**: This medium allows all bacteria to grow, including both normal bacteria and streptomycin-resistant mutants.
- **Medium with Streptomycin**: This medium contains streptomycin, which will only allow the growth of streptomycin-resistant mutants.

3. **Selecting the Right Medium**: To isolate streptomycin-resistant mutants, we need to use a medium that contains streptomycin. This is because only those bacteria that have developed resistance to streptomycin will be able to grow in its presence.

4. **Conclusion**: Therefore, to select streptomycin-resistant mutants, the imprints must be used on plates that contain streptomycin. This will ensure that only the resistant mutants are selected and can grow, while sensitive bacteria will be inhibited.

**Final Answer**: The imprints need to be used on plates with streptomycin.

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