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What do you understand by staggered ends...

What do you understand by staggered ends ?

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### Step-by-Step Solution: 1. **Definition of Staggered Ends**: Staggered ends refer to the ends of a DNA molecule that have been cut by restriction enzymes in such a way that they leave overhanging single-stranded regions. These ends are not blunt but rather have protruding segments. 2. **Mechanism of Cutting**: When DNA is cut by restriction endonucleases (enzymes that recognize specific sequences in the DNA), they do not always cut straight across the double helix. Instead, they may cut at different points on the two strands, resulting in staggered ends. 3. **Formation of Overhanging Regions**: The staggered cuts create overhanging regions, which are single-stranded DNA segments that extend beyond the double-stranded part of the DNA. These overhangs are complementary to each other, meaning that if one strand has a specific sequence, the other strand will have a corresponding sequence that can pair with it. ...
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