Method involving entry of high velocity gold microparticles coated with DNA into plant cell is

Options

A. Protoplast fusion
B. Transfection
C. Biolistics
D. Magic bullet action

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the method that involves the entry of high-velocity gold microparticles coated with DNA into plant cells.

2. **Analyzing the Options**:
- **Protoplast Fusion**: This method involves the fusion of plant cells that have had their cell walls removed (protoplasts). It does not involve gold microparticles or DNA coating, so this option is incorrect.
- **Transfection**: This term generally refers to the introduction of nucleic acids into cells using various methods. However, it does not specifically involve gold microparticles, making this option incorrect.
- **Biolistic Method**: This method, also known as the "gene gun" method, involves coating DNA onto gold or tungsten microparticles and then shooting them into plant cells at high velocity. This is a vectorless gene transfer method specifically used for plants, making this option correct.
- **Magic Bullet Action**: This term does not have a recognized meaning in biotechnology and is not related to the method described in the question, making this option incorrect.

3. **Conclusion**: The correct answer is the **biolistic method**, as it specifically describes the use of gold microparticles coated with DNA to transfer genetic material into plant cells.

**Final Answer**: The method involving entry of high-velocity gold microparticles coated with DNA into plant cells is the **biolistic method**.

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