Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is called

Options

A. Gene Gun method
B. Biolistic method
C. Microinjection method
D. Indirect method

Answer

To answer the question regarding the method in which recombinant DNA is directly injected into the nucleus of an animal cell using microneedles, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks for a specific method that involves the direct injection of recombinant DNA into the nucleus of an animal cell using microneedles.

2. **Identifying Key Terms**: The key terms in the question are "recombinant DNA," "injected," "nucleus," "animal cell," and "microneedles." These terms indicate a method of gene transfer.

3. **Reviewing Options**: The options provided are:
- Gene gun
- Biolistic
- Microinjection
- Indirect methods

4. **Analyzing Each Option**:
- **Gene Gun**: This method uses a device to shoot DNA into cells but does not involve microneedles.
- **Biolistic**: Similar to the gene gun, this method involves the delivery of nucleic acids but does not use microneedles.
- **Microinjection**: This method involves using a fine glass micropipette (acting as a microneedle) to inject DNA directly into the nucleus of a target cell.
- **Indirect Methods**: These methods do not involve direct injection into the target cells.

5. **Selecting the Correct Answer**: Based on the analysis, the method that directly injects recombinant DNA into the nucleus of an animal cell using microneedles is called **Microinjection**.

6. **Conclusion**: Therefore, the correct answer to the question is **Microinjection**.