Which one of the following techniques made it possible to gnetically engineer living organisms ?

Options

A. Heavier isotope labelling
B. Hybridization
C. Recombinant DNA techniques
D. X-ray difraction

Answer

To answer the question "Which one of the following techniques made it possible to genetically engineer living organisms?", we can follow these steps:

### Step 1: Understand the Techniques Listed
The options given are:
1. Heavier isotope labeling
2. Hybridization
3. Recombinant DNA technology
4. X-ray diffraction

### Step 2: Analyze Each Technique
- **Heavier isotope labeling**: This technique is primarily used for tracking and tracing molecules in biological systems, not for genetic engineering.

- **Hybridization**: This refers to the process of combining two complementary strands of nucleic acids. While it is useful in genetic analysis, it does not directly lead to the engineering of organisms.

- **Recombinant DNA technology**: This is a method that involves combining DNA from different sources. It allows scientists to insert genes of interest into plasmids (vectors), which can then be introduced into host organisms to produce desired proteins or traits.

- **X-ray diffraction**: This technique is used to determine the three-dimensional structures of molecules, including proteins and nucleic acids, but it does not involve genetic engineering.

### Step 3: Identify the Correct Answer
From the analysis, it is clear that the technique that enables the genetic engineering of living organisms is **Recombinant DNA technology**. This method allows for the manipulation of genetic material to produce desired traits or products in organisms.

### Final Answer
The technique that made it possible to genetically engineer living organisms is **Recombinant DNA technology**.

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