Which one of the following palindromic base sequences in DNA can be easily cut by EcoRI ?

Options

A. 5'……………CACGTA……………..3'
3'………….CTCAGT………….5'
B. 5'…………….CGTTCG…………..3'
3'………….ATGGTA………..5'
C. 5'…………..GATATG…………3'
3'……………CTACTA………..5'
D. 5'…………GAATTC………….3'
3'…………..CTTAAG……….5'

Answer

To determine which palindromic base sequence in DNA can be easily cut by EcoRI, we need to follow these steps:

### Step-by-Step Solution:

1. **Understand EcoRI's Recognition Sequence**:
EcoRI is a restriction enzyme that recognizes a specific palindromic sequence in DNA. The recognition sequence for EcoRI is:
- **5' - G A A T T C - 3'**
- **3' - C T T A A G - 5'**

2. **Identify Palindromic Sequences**:
A palindromic sequence is one that reads the same forwards and backwards on complementary strands. Therefore, we need to look for sequences that match EcoRI's recognition site.

3. **Check Given Options**:
If the question provides multiple sequences, we will check each one to see if it matches the EcoRI recognition sequence (G A A T T C).

4. **Select the Correct Sequence**:
The correct palindromic sequence that can be cut by EcoRI will be the one that matches G A A T T C on one strand and C T T A A G on the complementary strand.

5. **Conclusion**:
The sequence that matches EcoRI's recognition site is the one that can be easily cut by this enzyme.