Which is used in gene cloning

Options

A. Nucleoids
B. Lomasomes
C. Mesosomes
D. Plasmids

Answer

**Step-by-Step Solution:**

1. **Understanding Gene Cloning**: Gene cloning is a process where a specific gene is isolated and then inserted into a host organism to produce multiple copies of that gene. This is often done to produce proteins, such as insulin, in large quantities.

2. **Evaluating the Options**:
- **Option 1: Nucleotide**: Nucleotides are the building blocks of DNA and RNA. They are not used directly in gene cloning as we need the entire gene rather than just the individual nucleotides.
- **Option 2: Lysosome**: Lysosomes are organelles that contain enzymes for digestion within the cell. They are not involved in the process of gene cloning.
- **Option 3: Mesosome**: Mesosomes are infoldings of the bacterial cell membrane. They are not directly related to gene cloning processes.
- **Option 4: Plasmid**: Plasmids are small, circular pieces of DNA found in bacteria that can replicate independently. They are commonly used as vectors in gene cloning to carry the gene of interest into the host cell.

3. **Conclusion**: After evaluating all the options, the only suitable choice for gene cloning is **plasmid**. Plasmids serve as vectors to transfer the desired gene into a host organism, making it the correct answer.

**Final Answer**: Plasmid is used in gene cloning.

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