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A: Taq Polymerase is involved in PCR tec...

A: Taq Polymerase is involved in PCR technique.
R: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

A

If both Assertion & Reason are true and the reason is the correct explanation of the assertion

B

If both Assertion & Reason are true but the reason is not the correct explanation of the assertion

C

If Assertion is true statement but Reason is false

D

If both Assertion and Reason are false statements

Text Solution

AI Generated Solution

The correct Answer is:
### Step-by-Step Solution: 1. **Understanding the Assertion and Reason**: - The assertion states that Taq Polymerase is involved in the PCR technique. - The reason explains that this enzyme remains active during the high temperatures involved in the denaturation of double-stranded DNA. 2. **Explaining PCR Technique**: - PCR (Polymerase Chain Reaction) is a method used to amplify DNA. It involves three main steps: denaturation, annealing, and extension. 3. **Describing the Denaturation Step**: - In the denaturation step, the double-stranded DNA is heated to approximately 82 to 92 degrees Celsius, causing the two strands to separate. 4. **Introducing Taq Polymerase**: - Taq Polymerase is a specific type of DNA polymerase that is derived from the bacterium Thermus aquaticus, which lives in high-temperature environments. 5. **Highlighting the Importance of Taq Polymerase**: - Unlike regular DNA polymerases, Taq Polymerase is heat-stable. This means it can withstand the high temperatures used during the denaturation step without losing its activity. 6. **Connecting the Assertion and Reason**: - Since Taq Polymerase remains active during the high temperatures of the denaturation step, it is crucial for the PCR process. Thus, the assertion is true, and the reason correctly explains why Taq Polymerase is used in PCR. 7. **Conclusion**: - Both the assertion and the reason are true, and the reason is a correct explanation of the assertion. ### Final Answer: Both assertion and reason are true, and the reason is a correct explanation of the assertion. ---
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