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A: Ethidium bromide helps in visualizing...

A: Ethidium bromide helps in visualizing DNA in UV light only.
R: This intercalating agent absorbs light in range 260-330 nm.

A

If both Assertion & Reason are true and the reason is the correct explanation of the assertion

B

If both Assertion & Reason are true but the reason is not the correct explanation of the assertion

C

If Assertion is true statement but Reason is false

D

If both Assertion and Reason are false statements

Text Solution

AI Generated Solution

The correct Answer is:
To solve the question, we need to analyze the assertion and the reason provided: ### Step 1: Analyze the Assertion The assertion states: "Ethidium bromide helps in visualizing DNA in UV light only." - Ethidium bromide is a fluorescent dye that intercalates between the bases of DNA. - It emits an orange fluorescence when exposed to UV light, which allows for the visualization of DNA bands in gel electrophoresis. **Conclusion for Step 1**: The assertion is true. ### Step 2: Analyze the Reason The reason states: "This intercalating agent absorbs light in the range 260-330 nm." - Ethidium bromide indeed absorbs UV light in this range (260-330 nm), which is why it can fluoresce and allow for visualization of DNA. - This property of ethidium bromide as an intercalating agent is crucial for its function in DNA visualization. **Conclusion for Step 2**: The reason is also true and provides a correct explanation for the assertion. ### Step 3: Determine the Relationship Since both the assertion and the reason are true, and the reason correctly explains why the assertion is true, we conclude that the correct option is: **Answer**: Option A - Both assertion and reason are true, and reason is the correct explanation of assertion.
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