Mention the correct sequence of steps followed after separation of DNA fragments by electrophoresis in DNA fingerprinting.
(a) Hybridization.
(b) Autoradiography .
(c) Blotting.

Answer

To determine the correct sequence of steps followed after the separation of DNA fragments by electrophoresis in DNA fingerprinting, we can break down the process as follows:

### Step-by-Step Solution:

1. **Blotting (c)**: After the DNA fragments are separated by gel electrophoresis, the first step is blotting. This involves transferring the separated DNA fragments from the gel to a membrane, typically made of nitrocellulose or nylon. This step is crucial as it allows for the subsequent analysis of the DNA.

2. **Hybridization (a)**: Once the DNA fragments are transferred to the membrane, the next step is hybridization. In this step, specific DNA probes are used to bind to the target DNA fragments on the membrane. This allows for the identification of specific sequences within the DNA.

3. **Autoradiography (b)**: The final step is autoradiography. This technique is used to visualize the hybridized DNA probes. It involves exposing the membrane to X-ray film or a similar medium, which captures the signals from the labeled probes, revealing the presence and position of the DNA fragments.

### Correct Sequence:
The correct sequence of steps after the separation of DNA fragments by electrophoresis in DNA fingerprinting is:
1. **Blotting (c)**
2. **Hybridization (a)**
3. **Autoradiography (b)**

### Final Answer:
The correct order is: **c, a, b**.

---