During DNA fingerpriting, separation of DNA fragmemts is done by

Options

A. Autordiography
B. Hybridisation
C. Denaturation
D. Electrophoresis

Answer

**Step-by-Step Solution:**

1. **Understanding DNA Fingerprinting**: DNA fingerprinting is a technique used to identify individuals based on their unique DNA profiles. It is commonly used in forensic science to link biological evidence to suspects in criminal investigations.

2. **Process of DNA Fingerprinting**: The process of DNA fingerprinting involves several key steps:
- **Extraction**: DNA is extracted from the biological sample.
- **Quantitation**: The amount of DNA is quantified to ensure there is enough for analysis.
- **Amplification**: The DNA is amplified using techniques such as PCR (Polymerase Chain Reaction) to create enough copies for analysis.
- **Separation**: The final step involves separating the DNA fragments for analysis.

3. **Identifying the Separation Method**: The question asks about the method used for the separation of DNA fragments. The options provided are:
- Autoradiography
- Hybridization
- Denaturation
- Electrophoresis

4. **Choosing the Correct Method**: Among the options, electrophoresis is the correct method used for separating DNA fragments. Specifically, gel electrophoresis is a common technique where DNA fragments are separated based on their size.

5. **How Electrophoresis Works**: In gel electrophoresis:
- DNA samples are loaded into wells in a gel matrix.
- An electric current is applied, causing the negatively charged DNA fragments to migrate towards the positive electrode.
- Smaller DNA fragments move faster and farther through the gel than larger ones, resulting in separation based on size.

6. **Conclusion**: Therefore, during DNA fingerprinting, the separation of DNA fragments is done by electrophoresis (also known as gel electrophoresis).

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