Sequencing the whole set of genome that contained all the coding and non-coding sequence and later assigning different region in the sequences with functions are referred to as

Options

A. Sequence annotation
B. PCR
C. Northern blot
D. Microarray

Answer

To answer the question, "Sequencing the whole set of genome that contained all the coding and non-coding sequence and later assigning different regions in the sequences with functions are referred to as," we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Question**: The question is asking about a specific method of sequencing the entire genome, which includes both coding (genes) and non-coding sequences, and involves assigning functions to different regions of the genome.

2. **Identifying Key Terms**:
- **Whole Genome Sequencing**: This refers to sequencing the complete DNA of an organism.
- **Coding Sequences**: These are parts of the genome that contain the information necessary to produce proteins.
- **Non-Coding Sequences**: These are parts of the genome that do not code for proteins but may have regulatory or other functional roles.

3. **Recalling Relevant Projects**: The Human Genome Project (HGP) is a significant example of a project that aimed to sequence the entire human genome, including both coding and non-coding regions.

4. **Defining the Process**: The process of sequencing the entire genome and then assigning functions to various regions is known as **sequence annotation**. This involves identifying the roles of different sequences, whether they are coding for proteins or serving other functions.

5. **Eliminating Other Options**:
- **Express Sequence Tags (ESTs)**: This method focuses only on the coding regions and does not include non-coding sequences.
- **Polymerase Chain Reaction (PCR)**: This is a technique used to amplify DNA, not for sequencing the entire genome.
- **Northern Blot and Microarray**: These are techniques used for analyzing RNA and gene expression, not for whole genome sequencing.

6. **Final Answer**: Based on the above analysis, the correct term for the process described in the question is **sequence annotation**.