In the technique of DNA fingerprinting, digestion of DNA is followed by

Options

A. Electrophoresis
B. Hybridisation
C. Denaturation
D. Southern blotting

Answer

To answer the question regarding what follows the digestion of DNA in the technique of DNA fingerprinting, we can break down the process step by step.

### Step-by-Step Solution:

1. **Understanding DNA Digestion**:
- In DNA fingerprinting, the first step involves the digestion of DNA using restriction enzymes (also known as restriction endonucleases). These enzymes cut the DNA at specific sequences, resulting in smaller fragments.

2. **Electrophoresis**:
- After the DNA has been digested into fragments, the next step is to separate these fragments based on their size. This is done using a technique called gel electrophoresis. In this process, the DNA fragments are loaded into a gel matrix and an electric current is applied. Since DNA is negatively charged, the fragments will migrate towards the positive electrode (anode).

3. **Hybridization**:
- Once the DNA fragments are separated, hybridization may occur. This involves the use of labeled probes that can bind to specific DNA sequences. However, this step is not directly after digestion but is part of the overall process of analyzing the fragments.

4. **Denaturation**:
- Denaturation typically refers to the process of separating double-stranded DNA into single strands, often using heat or chemicals like sodium hydroxide. This step is also not directly after digestion but may occur later in the analysis.

5. **Southern Blotting**:
- After electrophoresis, the next step is often Southern blotting. This technique transfers the separated DNA fragments from the gel onto a membrane, allowing for further analysis and detection of specific sequences using probes.

### Conclusion:
In the context of the question, after the digestion of DNA, the next process is **Electrophoresis**. This step is crucial for separating the DNA fragments before any further analysis like hybridization or Southern blotting.

### Final Answer:
**Electrophoresis**

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