In-vitro template independent RNA synthesis is a feature of

Options

A. RNA polymerase
B. Reverse transcriptase
C. Ochoa enzyme
D. DNA polymerase

Answer

To solve the question regarding in-vitro template independent RNA synthesis, we can follow these steps:

### Step 1: Understand the Question
The question asks about which enzyme is responsible for in-vitro template independent RNA synthesis. This means we are looking for an enzyme that can synthesize RNA without needing a DNA or RNA template.

### Step 2: Analyze the Options
The options provided are:
- A) RNA polymerase
- B) Reverse transcriptase
- C) Okoye enzyme
- D) DNA polymerase

### Step 3: Evaluate Each Option
1. **RNA Polymerase**: This enzyme synthesizes RNA from a DNA template. Therefore, it does not fit the criteria of being template independent.

2. **Reverse Transcriptase**: This enzyme synthesizes DNA from an RNA template. Again, it requires a template, so it is not the correct answer.

3. **Okoye Enzyme (Polynucleotide Phosphorylase)**: This enzyme can synthesize RNA in a template-independent manner. It uses ribonucleoside diphosphates instead of ribonucleoside triphosphates, making it unique in its functionality.

4. **DNA Polymerase**: This enzyme synthesizes DNA from a DNA template, thus it also does not meet the criteria of being template independent.

### Step 4: Conclusion
Based on the analysis, the correct answer is **C) Okoye enzyme**. This enzyme is known for its ability to catalyze RNA synthesis without the need for a template.