Termination of polypeptide synthesis in bacteria differs from eukaryotes in

Options

A. Having different termination codons
B. Being GTP dependent
C. Involving more than one type of release factors
D. All of these

Answer

### Step-by-Step Solution:

1. **Understanding the Context**:
- The question asks about the differences in the termination of polypeptide synthesis between bacteria (prokaryotes) and eukaryotes.

2. **Polycistronic vs. Monocistronic mRNA**:
- In prokaryotes (bacteria), mRNA is often polycistronic, meaning it can code for multiple polypeptides.
- In contrast, eukaryotic mRNA is monocistronic, coding for a single polypeptide.

3. **Termination Codons**:
- Prokaryotes have three termination codons: UAA, UAG, and UGA.
- Eukaryotes typically have a single termination codon.

4. **Release Factors**:
- Prokaryotes utilize multiple release factors for termination. Specifically, they have three release factors (RF1, RF2, and RF3) that recognize the stop codons and facilitate the termination process.
- Eukaryotes generally use a single release factor for termination.

5. **GTP Dependency**:
- Both prokaryotes and eukaryotes require GTP (Guanosine Triphosphate) for the termination of translation. GTP is essential for the function of the release factors and the overall process of protein synthesis.

6. **Conclusion**:
- The termination of polypeptide synthesis in bacteria differs from eukaryotes in three main ways:
1. The presence of multiple termination codons in bacteria.
2. The involvement of multiple release factors in bacteria.
3. The GTP dependency is common to both but is crucial in the termination process.

### Final Answer:
The termination of polypeptide synthesis in bacteria differs from eukaryotes in that bacteria have multiple termination codons, involve multiple release factors, and are GTP dependent.

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