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The DNA strand showing replication using...

The DNA strand showing replication using Okazaki fragments also shows.

A

Continuous growth in `5 to 3` direction

B

Discontinuous growth on `5 to 3` parental strand

C

Discontinuous growth on `3 to 5` parental strand

D

Involvement of one primer only

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To solve the question regarding the DNA strand showing replication using Okazaki fragments, we will follow these steps: ### Step-by-Step Solution: 1. **Understanding DNA Replication**: - DNA replication occurs at the replication fork where the double helix unwinds. There are two strands: the leading strand and the lagging strand (which is composed of Okazaki fragments). 2. **Identifying the Leading and Lagging Strands**: - The leading strand is synthesized continuously in the 5' to 3' direction. - The lagging strand is synthesized discontinuously in short segments known as Okazaki fragments, which are formed in the 5' to 3' direction but are laid down in the opposite direction to the overall direction of replication. 3. **Directionality of the Strands**: - The parental strand is oriented in the 3' to 5' direction for the lagging strand (Okazaki fragments). This means that the Okazaki fragments are synthesized in the 5' to 3' direction, but they are formed in a discontinuous manner. 4. **Analyzing the Options**: - **Continuous growth in the 5' to 3' direction**: This refers to the leading strand, not the Okazaki fragments. - **Discontinuous growth in the 5' to 3' parental strand**: This is incorrect because the Okazaki fragments grow in the 5' to 3' direction but are not continuous. - **Discontinuous growth on the 3' to 5' parental strand**: This is correct as the Okazaki fragments are synthesized on the lagging strand, which is complementary to the 3' to 5' parental strand. - **Involvement of one primer only**: This is incorrect as each Okazaki fragment requires its own RNA primer for initiation. 5. **Conclusion**: - The correct answer is that the DNA strand showing replication using Okazaki fragments also shows **discontinuous growth on the 3' to 5' parental strand**. ### Final Answer: The DNA strand showing replication using Okazaki fragments also shows discontinuous growth on the 3' to 5' parental strand. ---
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Consider the following events in DNA replication 1. Formation of RNA primers by RNA polymerase. 2. Removal of RNA primers and formation of Okazaki fragments. Formation of DNA strands on RNA primers by DNA polyIII complex. 4. Recognition of intiation point by initiator protein. 5. Dissociation of hydrogen bonds and opening of a 'bubbble' in the duplex by unwinding proteins. 6. Filling up of the gaps between Okazaki fragments and formation of DNA strand. The correct sequence of these events is:-

A 3' ______________ 5' B C 5' _______________ 3'D AB and CD represent two strands of a DNA molecule. When this molecule undergoes replication, forming a replication fork between A and C in the above. (i) Name the template strands for replication. (ii) Using which strand as the template, will there be continuous synthesis of a complementary DNA strand? (iii) Complementary to which strand will Okazaki segments get synthesised and discontinuous synthesis will occur (iv) What are template strands and Okazaki pieces? (v) In which direction is a new strand synthesized?

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