One of the most frequently used techniques in DNA fingerprinting is

Options

A. AFLP
B. VNTR
C. SSCP
D. SCAR

Answer

### Step-by-Step Solution:

1. **Understanding DNA Fingerprinting**:
- DNA fingerprinting is a technique used to identify individuals based on their unique DNA profiles. It is widely utilized in forensic science, paternity testing, and genetic research.

2. **Analyzing the Options**:
- The question provides four options: AFLP, VNTR, SSCP, and SCAR. We need to determine which of these techniques is most frequently used in DNA fingerprinting.

3. **Evaluating Each Option**:
- **AFLP (Amplified Fragment Length Polymorphism)**: This technique is indeed a PCR-based method that generates unique DNA profiles by amplifying specific fragments of DNA. It is commonly used in DNA fingerprinting.
- **VNTR (Variable Number of Tandem Repeats)**: While VNTRs are important in genetic analysis, they are not the most frequently used technique in DNA fingerprinting.
- **SSCP (Single-Stranded Confirmation Polymorphism)**: This method is primarily used for detecting mutations rather than for DNA fingerprinting.
- **SCAR (Sequence Categorized Amplified Reaction)**: This technique is used for genetic purity testing and is not primarily associated with DNA fingerprinting.

4. **Conclusion**:
- After evaluating all the options, it is clear that **AFLP** is the most frequently used technique in DNA fingerprinting due to its ability to generate and compare unique DNA profiles effectively.

### Final Answer:
The most frequently used technique in DNA fingerprinting is **AFLP (Amplified Fragment Length Polymorphism)**.

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