Home
Class 12
BIOLOGY
Choose the correct sequence of steps use...

Choose the correct sequence of steps used in DNA fingerprinting d. Agarose gel electrophoresis, a. DNA extraction e. Collection of a biological sample- blood, saliva, buccal swab, semen, or solid tissue. c. Interpreting results b. Restriction digestion or PCR amplification

A

`e to a to b to d to c`

B

`e to a to b to d to c`

C

`e to d to a to b to c`

D

`e to a to d to b to c`

Text Solution

Verified by Experts

Promotional Banner

Similar Questions

Explore conceptually related problems

Mention the correct sequence of steps followed after separation of DNA fragments by electrophoresis in DNA fingerprinting. (a) Hybridization. (b) Autoradiography . (c) Blotting.

Read the following statements regarding agarose gel electrophoresis and select the CORRECT ones. I. The longest DNA fragments are nearest to the anode. II. The matrix of the gel is a natural polymer obtained from seaweeds. III The process is used to check the progression of restriction enzyme digestion. IV. The fragments of DNA separated due to the sieving effect provided by agarose gel.

Arrange the various steps of DNA fingerprinting technique in the correct order. (i) Separation of DNA fragments by electrophoresis. (ii) Digestion of DNA by restriction endonucleases. (iii) Hybridisation using labelled VNTR prode. (iv) Isolation of DNA . Detection of hybridised DNA fragments by auto-radiography. (vi) Transferring the separated DNA fragments to nitrocellulose membrane.

Given below are the steps of DNA fingerprinting in random sequence:- (i) Isolation of DNA. (ii) Separation of DNA fragements by electrophoresis, (iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon. (v) digestion of DNA by restriction endonucleases, (vi). Detection of hybridised DNA fragments by auto radio-graphy. which of the following is the correct sequence:-

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below

Some of the steps of DNA fingerprinting are given below. Identify the correct sequence from the option given: A. Electrophoresis of DNA fragments B. Hybridisation with DNA probe C. Digestion of DNA by RENs D. Autoradiography E. Blotting of DNA fragments to nitrocellulose membrane

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Which of the following are correct? a) PCRs are defined as the organisms, which carry foreign genes b) SCID is a group of rare genetic disorders c) Cloning is the production of GMOs, eg, Flavr-savr tomato d) The bacteria use restriction endonucleases to destroy the DNA of invading foreign viruses

Read the given four statements and choose the correct option. a. An alien DNA is linked with the "ori" for multiplication b. Normal E.coli cells do not carry antibiotic resistant genes for tetracycline and ampicilin c. Salmonella typhimurium carry gene for resistance against antibiotic d. An antibiotic resistance gene can be used as a selectable marker or cloning site

A DNA fragement was cleaved by using REN and the restriction fragments ( a,b and c ) produced as a result of digestion are shown below. Select the option that represents the correct gel electrophoresis separation of these fragments.