______ help to stop substances from leaking across a tissue.Choose the option which files the blank correctly.

Options

A. Gap junctions
B. Adhering junctions
C. Tight junctions
D. Interdigitations

Answer

To solve the question "______ help to stop substances from leaking across a tissue," we need to identify the correct type of cellular junction that serves this function.

### Step-by-Step Solution:

1. **Understand the Options**: The options provided are:
- Gap junction
- Adhering junction
- Tight junction
- Interdigitation

2. **Analyze Each Option**:
- **Gap Junction**: These are specialized intercellular connections that allow the passage of ions and small molecules between adjacent cells. They do not prevent leakage; instead, they facilitate communication.
- **Adhering Junction**: These junctions help to keep neighboring cells together but do not specifically prevent leakage of substances across tissues.
- **Tight Junction**: The primary function of tight junctions is to prevent substances from leaking between cells, effectively creating a barrier that maintains the integrity of the tissue.
- **Interdigitation**: This refers to the interlocking of cell membranes, which increases surface area for adhesion but does not primarily function to prevent leakage.

3. **Identify the Correct Answer**: Based on the analysis, the option that correctly fills the blank is **Tight Junction**. Tight junctions are crucial for maintaining the selective permeability of epithelial tissues and preventing leakage of substances.

4. **Final Answer**: Therefore, the correct answer to the question is **Tight Junction**.