Step catalyzed by pacemaker enzyme of EMP pathway is
(a) Phosphorylation of Glucose-6-phosphate to Fructose-6-phosphate
(b) Phosphorylation of Fructose-6-phosphate to Fructose-1-6-bisphosphate
(c) Phosphorylation of triose phosphate to triose bisphosphate
(d) Splitting of Fructose 1-6-bisphosphate

Options

A. Phosphorylation of Glucose-6-phosphate to Fructose-6-phosphate
B. Phosphorylation of Fructose-6-phosphate to Fructose-1-6-bisphosphate
C. Phosphorylation of triose phosphate to triose bisphosphate
D. Splitting of Fructose 1-6-bisphosphate

Answer

To solve the question regarding the step catalyzed by the pacemaker enzyme of the EMP pathway, we will analyze each option provided and determine which one corresponds to the role of the pacemaker enzyme in glycolysis.

### Step-by-Step Solution:

1. **Understanding the EMP Pathway**:
- The EMP pathway, also known as the Embden-Meyerhof-Parnas pathway, is synonymous with glycolysis. It is the metabolic pathway that converts glucose into pyruvate, generating ATP and NADH in the process.

2. **Identifying the Pacemaker Enzyme**:
- The pacemaker enzyme in glycolysis is known as phosphofructokinase (PFK). This enzyme is crucial because it regulates the rate of glycolysis. It is responsible for one of the key regulatory steps in the pathway.

3. **Analyzing the Options**:
- **(a)** Phosphorylation of Glucose-6-phosphate to Fructose-6-phosphate: This step is not catalyzed by the pacemaker enzyme.
- **(b)** Phosphorylation of Fructose-6-phosphate to Fructose-1,6-bisphosphate: This step is catalyzed by phosphofructokinase and is indeed the rate-limiting step in glycolysis.
- **(c)** Phosphorylation of triose phosphate to triose bisphosphate: This is not a step catalyzed by the pacemaker enzyme.
- **(d)** Splitting of Fructose 1,6-bisphosphate: This is catalyzed by aldolase, not by the pacemaker enzyme.

4. **Conclusion**:
- Based on the analysis, the correct answer is **(b)** Phosphorylation of Fructose-6-phosphate to Fructose-1,6-bisphosphate, as this is the step catalyzed by the pacemaker enzyme (phosphofructokinase) in the EMP pathway.

### Final Answer:
**(b) Phosphorylation of Fructose-6-phosphate to Fructose-1,6-bisphosphate.**