Choose incorrect match amongst restriction enzymes listed in column l and type of ends produced in column ll

Options

A. Column I EcoR I and Column II Sticky end
B. Column I - Hind III and Column II - Sticky end
C. Column I - Sma I and Column II - Blunt end
D. Column I - Bam HI and Column II - Flush end

Answer

To solve the question of identifying the incorrect match between restriction enzymes and the types of ends they produce, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Types of Ends**:
- There are two main types of ends produced by restriction enzymes when they cut DNA:
- **Sticky Ends**: These are overhanging ends that can easily pair with complementary DNA strands.
- **Blunt Ends (Flush Ends)**: These ends are straight cuts with no overhang.

2. **List the Enzymes and Their Corresponding Ends**:
- We need to evaluate each enzyme listed in Column I and match it with the type of end it produces in Column II.

3. **Evaluate Each Match**:
- **E. coli (EcoRI)**: Produces sticky ends.
- **Match**: Correct.
- **HindIII**: Produces sticky ends.
- **Match**: Correct.
- **SmaI**: Produces blunt ends.
- **Match**: Correct.
- **BamHI**: The question states it produces blunt ends (flush ends).
- **Match**: Incorrect. BamHI actually produces sticky ends.

4. **Identify the Incorrect Match**:
- Based on the evaluation, the incorrect match is **BamHI** producing **blunt ends**. The correct type of end produced by BamHI is **sticky ends**.

5. **Conclusion**:
- The answer to the question is that the incorrect match is **D: BamHI - Blunt Ends**.