Which of the following restriction enzyme site is present in nucleotide sequence of ampicillin resistance gene in pBR322?

Options

A. Pvu I
B. Pvu II
C. Hind III
D. Sal I

Answer

To solve the question regarding which restriction enzyme site is present in the nucleotide sequence of the ampicillin resistance gene in pBR322, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: pBR322 is a plasmid commonly used in genetic engineering. It contains genes for antibiotic resistance, specifically ampicillin and tetracycline.

2. **Identify the Genes**: The plasmid has two main antibiotic resistance genes:
- **Ampicillin Resistance Gene (APR)**
- **Tetracycline Resistance Gene (TET)**

3. **Know the Restriction Enzymes**: The options provided are:
- PVU1
- PVU2
- HIN3
- SAL1

4. **Analyze the Restriction Sites**:
- **PVU1**: This restriction enzyme site is present in the ampicillin resistance gene of pBR322.
- **PVU2**: This restriction enzyme site is not present in the ampicillin resistance gene.
- **HIN3**: This enzyme site is also not present in the ampicillin resistance gene; it is located between the ampicillin and tetracycline resistance genes.
- **SAL1**: This restriction enzyme site is found in the tetracycline resistance gene, not in the ampicillin resistance gene.

5. **Conclusion**: Based on the analysis, the only restriction enzyme site that is present in the nucleotide sequence of the ampicillin resistance gene in pBR322 is **PVU1**.

### Final Answer:
The correct option is **PVU1**.

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