Home
Class 12
BIOLOGY
Type - II restriction enzymes...

Type - II restriction enzymes

Text Solution

Verified by Experts

Two enzymes responsible for restricting the growth of Bacteriophage in Escherichia coli were isolated in the year 1963. One of these added methyl groups to DNA and the other cut DNA. The latter was called restriction endonuclease. The first restriction endonuclease - Hind II which cut DNA molecules at a particular point by recognising a specific sequence of six base pairs, called recognition sequence for Hind II. Today, more than 900 restriction enzymes were isolated from over 200 strains of Bacteria, each of which recognises a different recognition sequence.
E CORI is a restriction enzyme in which, the first letter comes from the Genus (Escherichia), and the second two letters from the species of the Prokaryotic cell [coli], the letter .R. is derived from the name of strain. Roman numbers indicate the order in which the enzymes were isolated from that strain of Bacteria. Restriction enzymes belong to a larger class of enzymes called nucleases. They are of two types.
a) Exonucleases which remove nucleotides from the ends of the DNA.
b) Endonucleases which make cuts at specific location with in the DNA.
Doubtnut Promotions Banner Mobile Dark
|

Topper's Solved these Questions

  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    VIKRAM PUBLICATION ( ANDHRA PUBLICATION)|Exercise LONG ANSWER QUESTION |2 Videos

  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    VIKRAM PUBLICATION ( ANDHRA PUBLICATION)|Exercise EXERCISE |20 Videos

  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    VIKRAM PUBLICATION ( ANDHRA PUBLICATION)|Exercise IMPORTANT QUESTIONS |12 Videos

  • BACTERIA

    VIKRAM PUBLICATION ( ANDHRA PUBLICATION)|Exercise Important question|9 Videos

  • BIOTHECHNOLOGY AND IT'S APPLICATIONS

    VIKRAM PUBLICATION ( ANDHRA PUBLICATION)|Exercise EXERCISE|6 Videos

Similar Questions

Explore conceptually related problems

Write short notes on restriction enzymes:

Explain briefly (a) PCR (b) Restriction enzymes and DNA (c) Chitinase

Knowledge Check

  • Match the following terms in Column-I with their descriptions by writing the appropriate letters from Column-II. Column-I A) A molecule used to carry foreign B) A rapid way to amplify DNA C) A way to separate DNA fragments D) Molecular scissors E) A gene sequence from more than one origin Column - II I) Restriction enzymes genes into bacteria II) Recombinant DNA in the laboratory III) PCR based on their size IV) Vector V) Gel electrophoresis

    A
    `{:(A,B,C,D,E),(IV,III,V,I,II):}`
    B
    `{:(A,B,C,D,E),(V,I,III,II,IV):}`
    C
    `{:(A,B,C,D,E),(V,I,III,II,IV):}`
    D
    `{:(A,B,C,D,E),(II,V,IV,I,III):}`

  • Cleavage style of restriction enzymes includes

    A
    blunt end style
    B
    sticky end style
    C
    no style
    D
    Both (1) and (2)

  • Match each term on the right with the definition on the left, Column - I A) Bacterial enzymes used to cut DNA at defined sequences B) Sequences cut by restriction enzymes C) Ends left on DNA segments cut by DNA restriction enzymes D) Circular pieces of DNA found in bacteria E) Bacterial viruses F) Process by which bacteria take up pieces of DNA from the environment G) Plasmid DNA that has incorporated human DNA H) Term used to describe any vehicle that moves DNA from the organism to another Column - II I) Transformation II) Cloning vector III) Recombinant IV) Recognition sequences V) Plasmids VI) Sticky ends VII) Restriction enzymes VIII) Bacteriophages

    A
    A-VII,B-IV,C-VI,DI-V,E-VIII,F-I,G-III,H-II`
    B
    A-IV,B-VI,C-I,D-III,E-V,F-VII,G-VIII,H-II
    C
    A-III,B-V,C-VII,D-VIII,E-II,F-IV,G-VI,H-I
    D
    A-IV,B-III,C-V,D-VI,E-VII,F-I,G-VIII,H-II

    • Similar Questions

    Explore conceptually related problems

    More thean 900 restriction enzymes have been isolated from

    Generally the palindromic sequence in dsDNA at which restriction enzymes act and cut into small segments contain

    A plasmid has two antibiotic-resistance genes, one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the ones that

    The restriction enzyme EcoRI recognize following palindrome sequence.

    Which of the following could be restriction enzyme recognition site ?

    Home
    Profile