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What are the physical barriers of a cell...

What are the physical barriers of a cell in the protoplast fusion experiment ? How are the barriers overcome ?

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Cell wall is the physical barrier of cell. To isolate protoplast from the cells, the cells are to be treated with cellulages and pectinages to break the cell walls. Then protoplasts are taken and are fused with the help of polyethylene glycol to obtain a hybrid plants.
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