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An amino acid is characterized by two pK...

An amino acid is characterized by two `pKa` values the one corresponding to the more acidic site is designated as `pKa_(1)` and the other corresponding to the less site is designated as `pKa_(2)`. Some amino acids have side chain with acidic or basic groups. These amino acids have `pKa_(3)` value also for the side chain.
`|{:(S.No,"Amino acid",P^(Ka1),P^(Ka2),P^(Ka3)" (Side chain)"),(I,"Aspartic acid",1.88,9.6,3.65),(II,"Glutamic acid",2.19,9.67,4.25),(III,"Lysine",2.18,8.95,10.53),(IV,"Arginine",2.17,9.04,12.48):}|`
The isoelectric point `(pI)` of Aspartic acid and lysine will be respectively `:`

A

6.35

B

9.74

C

2.77

D

10.76

Text Solution

Verified by Experts

The correct Answer is:
B

for basic amino acids
`P^1=(P^(Ka_2)+P^(ka_3))/2=(8.95+10.53)/2=19.48/2=9.74`
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An amino acid is characterized by two pKa values the one corresponding to the more acidic site is designated as pKa_1 and the other corresponding to the less acidic site is designated as pKa_2 The isoelectric point also called isoionic point (pI) is the pH at which concentration of zwitter ion is maximum . pI is the average of pKa_1 and pKa_2 .Generally the value of pI is slightly less than 7. some amino acids have side chain with acidic or basic groups.These amino acids have pKa_3 value also for the side chain.Acidic amino acid have acidic side chains and basic amino acids have basic side chains . pI for acidic amino acid is average of pKa_1 and pKa_3 .pI for basic amino acid is the average of pKa_2 and pKa_3 . {:(S.NO,"Amino acid",p^(Ka1),p^(Ka2),p^(Ka3)("side chain")),(I,"Aspartic acid",1.88,9.6,3.65),(II,"Glutamic acid",2.19,9.67,4.25),(III,"Lysine",2.18,8.95,10.53),(IV,"Arginine",2.17,9.04,12.48):} In the table given above the acidic amino acids are

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