During DNA replication, the separated strands of DNA are prevented from recoiling by

Options

A. DNA primase
B. Sigma factor
C. Rho-factor
D. SSBP

Answer

**Step-by-Step Solution:**

1. **Understanding DNA Replication**: During DNA replication, the double helix structure of DNA unwinds to allow the synthesis of new strands. This unwinding creates two separate strands of DNA.

2. **Role of Helicase**: The enzyme helicase is responsible for unwinding the DNA strands. It breaks the hydrogen bonds between the base pairs, separating the two strands.

3. **Preventing Recoiling**: Once the strands are separated, they have a tendency to recoil back into their original double-helix structure. To prevent this recoiling, additional mechanisms are needed.

4. **Function of Single-Stranded Binding Proteins (SSBPs)**: Single-stranded binding proteins (SSBPs) bind to the separated strands of DNA. Their primary role is to stabilize these single strands and prevent them from re-annealing or recoiling.

5. **Conclusion**: Therefore, during DNA replication, the separated strands of DNA are prevented from recoiling by single-stranded binding proteins (SSBPs).

**Final Answer**: The correct option is single-stranded binding proteins (SSBPs).

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