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The fluorescent dye used in agarose gel ...

The fluorescent dye used in agarose gel electrophoresis.

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Gel electrophoresis is a

Explain electrophoresis.

A plasmid DNA and a linear DNA (both of the same size) have one site for a restriction endonuclease, When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while, linear DNA shows two fragments. Explain.

Write brief notes on the Agarose gel electrophoresis.

Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

The anionic detergent, used in polyacrylamide gel electrophoresis is known as ……………………. .

MODERN PUBLICATION-PRINCIPLE AND PROCESS OF BIOTECHNOLOGY-EXERCIS
  1. The DNA digesting enzyme that removes nucleotides from the termini.

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  2. The enzyme that catalyses the synthesis of RNA on a DNA template.

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  3. The fluorescent dye used in agarose gel electrophoresis.

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  4. Name the technique used for the separation of DNA fragments.

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  5. The restriction endonuclease isolated from Escherichia coli .

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  6. Transfer of DNA fragments from the agarose gel to a nylon membrane.

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  7. The instrument used in PCR amplification.

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  8. You have chosen a plasmid as vector for cloning your gene. However, th...

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  9. Enzymes that cut the DNA at specific sites are called.

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  10. The enzyme required for making genes from the mRNA is called .

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  11. The plasmid of A. tumefacien is called .

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  12. Plants possessing genes of other organisms are called plants.

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  13. are most widely used cloning vectors in genetic engineering.

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  14. Short DNA molecules with known base sequences used for recognition of ...

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  15. As transgenic plants and animals produce warious useful substances, th...

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  16. Bt gene is transferred to plants from the bacterium

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  17. Seeds which cannot tolerate drying and freezing tempetaure are calleds...

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  18. enzymes join the cut ends of DNA.

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  19. Institutions for collection and maintenance of germplasm are called

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  20. The word 'biotechnology' was coined by ………………...

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