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The restriction endonuclease isolated fr...

The restriction endonuclease isolated from Escherichia coli .

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Escherichia coli is

The first restriction endonuclease isolated was

Restriction endonuclease are used as:

Restriction endonuclease is employed for cutting :

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (ii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector facilitates the gene cloning.

MODERN PUBLICATION-PRINCIPLE AND PROCESS OF BIOTECHNOLOGY-EXERCIS
  1. The fluorescent dye used in agarose gel electrophoresis.

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  2. Name the technique used for the separation of DNA fragments.

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  3. The restriction endonuclease isolated from Escherichia coli .

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  4. Transfer of DNA fragments from the agarose gel to a nylon membrane.

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  5. The instrument used in PCR amplification.

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  6. You have chosen a plasmid as vector for cloning your gene. However, th...

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  7. Enzymes that cut the DNA at specific sites are called.

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  8. The enzyme required for making genes from the mRNA is called .

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  9. The plasmid of A. tumefacien is called .

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  10. Plants possessing genes of other organisms are called plants.

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  11. are most widely used cloning vectors in genetic engineering.

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  12. Short DNA molecules with known base sequences used for recognition of ...

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  13. As transgenic plants and animals produce warious useful substances, th...

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  14. Bt gene is transferred to plants from the bacterium

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  15. Seeds which cannot tolerate drying and freezing tempetaure are calleds...

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  16. enzymes join the cut ends of DNA.

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  17. Institutions for collection and maintenance of germplasm are called

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  18. The word 'biotechnology' was coined by ………………...

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  19. Class II restriction endonucleases (enzymes) recognise specific nucleo...

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  20. Cohesive ends in the DNA fragments are generated by ........... cuttin...

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