Select the correct sequence for gram staining procedure.

Options

A. Gentian violet, Alcohol, Iodine solution, Safranin
B. Crystal violet, KI solution , Alcohol,Safranin
C. Crystal violet, Safranin , KI solution , Alcohol
D. Crystal violet, Alcohol, KI solution, Safranin

Answer

To answer the question regarding the correct sequence for the gram staining procedure, we can break down the process into clear steps:

### Step-by-Step Solution:

1. **Crystal Violet Application**: The first step in the gram staining procedure is to apply crystal violet to the bacterial smear. This is the primary stain that imparts a blue color to the cells.

2. **Iodine Treatment**: After the crystal violet, the smear is treated with iodine. Iodine acts as a mordant, which helps to fix the crystal violet dye within the bacterial cells.

3. **Decolorization**: The next step involves using a decolorizer, typically a mixture of alcohol and acetone. This step is crucial as it differentiates between gram-positive and gram-negative bacteria. Gram-positive bacteria retain the crystal violet color, while gram-negative bacteria lose it.

4. **Counterstaining with Safranin**: Finally, a counterstain, usually safranin, is applied. This red dye will color the gram-negative bacteria that have lost the crystal violet, while the gram-positive bacteria remain purple.

### Correct Sequence:
- Crystal Violet → Iodine → Alcohol → Safranin

### Final Answer:
The correct sequence for the gram staining procedure is: **Crystal Violet, Iodine, Alcohol, Safranin**.

---