In Gram staining technique the bacteria are stained with :

Options

A. Weakly alkaline silution of crystal violet or gentain violet
B. Strong alkaline solution of crystal violet or gentian violet.
C. Basic feulgen stain
D. Janus green B

Answer

### Step-by-Step Solution:

1. **Understanding Gram Staining**: The Gram staining technique is a method used to classify bacteria into two groups: Gram-positive and Gram-negative. This classification is based on the composition of the bacterial cell wall.

2. **Identifying the Stain Used**: The primary stain used in the Gram staining process is a weakly alkaline solution of crystal violet or gentian violet. This stain is crucial for differentiating between the two types of bacteria.

3. **Mechanism of Staining**:
- Gram-positive bacteria have a thick layer of peptidoglycan in their cell walls, which retains the purple color of the crystal violet stain.
- Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane, which does not retain the purple color after decolorization.

4. **Counter Staining**: After the decolorization step, Gram-negative bacteria are stained with a counterstain, typically safranin, which gives them a pink color.

5. **Conclusion**: Therefore, the correct answer to the question is that in the Gram staining technique, the bacteria are stained with a weakly alkaline solution of crystal violet or gentian violet.

### Final Answer:
In Gram staining technique, the bacteria are stained with a weakly alkaline solution of crystal violet or gentian violet.

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