Identify the restriction endonuclease that will provide sticky ends of DNA strands :

Options

A. Hind II
B. Sma I
C. Eco RI
D. Hae III

Answer

To identify the restriction endonuclease that provides sticky ends of DNA strands, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Function of Restriction Endonucleases**:
- Restriction endonucleases are enzymes that cut DNA at specific sequences. They can create either sticky ends or blunt ends depending on how they cut the DNA.

2. **Identify the Options**:
- The options given are:
- Option 1: HIND II
- Option 2: SMI (or SMI1)
- Option 3: Eco R1
- Option 4: Hae III

3. **Analyze Each Option**:
- **HIND II**: This enzyme does not produce sticky ends; it creates blunt ends.
- **SMI (SMI1)**: Similar to HIND II, SMI also produces blunt ends and does not create sticky ends.
- **Eco R1**: This enzyme is known for cutting DNA in a way that produces sticky ends, making it a suitable candidate.
- **Hae III**: Like HIND II and SMI, Hae III also produces blunt ends.

4. **Determine the Correct Answer**:
- Based on the analysis, Eco R1 is the only restriction endonuclease among the options that provides sticky ends of DNA strands.

5. **Final Conclusion**:
- The restriction endonuclease that provides sticky ends of DNA strands is **Eco R1**.