Which technique of DNA amplification was developed as a result of the work of Kary Mullis?

Options

A. ELISA
B. r-DNA technology
C. PCR
D. Genetic engineering

Answer

**Step-by-Step Solution:**

1. **Understanding DNA Amplification**: DNA amplification refers to the process of generating multiple copies of a specific DNA segment. This is essential in various applications, such as forensic analysis, medical diagnostics, and research.

2. **Identifying the Purpose of DNA Amplification**: The need for amplifying DNA arises in situations where only a small amount of DNA is available. For instance, in criminal investigations, a tiny DNA fragment found at a crime scene may need to be amplified for further analysis.

3. **Recognizing the Techniques for DNA Amplification**: Several techniques exist for DNA amplification, but one of the most significant and widely used methods is the Polymerase Chain Reaction (PCR).

4. **Linking PCR to Kary Mullis**: PCR, which stands for Polymerase Chain Reaction, was developed by Kary Mullis in the 1980s. This technique allows for the exponential amplification of a specific DNA sequence, making it easier to study or analyze.

5. **Evaluating Other Options**:
- **ELISA** (Enzyme-Linked Immunosorbent Assay) is unrelated to DNA amplification and was developed by other scientists.
- **rDNA Technology** (Recombinant DNA Technology) and **Genetic Engineering** are related but were developed by different scientists (Boyer and Cohen) and do not specifically refer to the amplification of DNA.

6. **Conclusion**: Based on the information provided, the technique of DNA amplification developed as a result of Kary Mullis's work is PCR.

**Final Answer**: PCR (Polymerase Chain Reaction)

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