What is not true w.r.t. the method of gene transfer by Gene Gun method?

Options

A. A.Tungsten or gold particles are used
B. B. The technique uses a micromanipulator to inject DNA
C. C. Also known as biolistics
D. D. Both (2) and (3)

Answer

**Step-by-Step Solution:**

1. **Understanding the Gene Gun Method**: The gene gun method, also known as the biolistic method, is a technique used for gene transfer, particularly in plant cells. It involves bombarding plant cells with high-velocity microparticles coated with DNA.

2. **Analyzing the Options**:
- **Option A**: "Tungsten or gold particles are used." This statement is true as the gene gun method uses these materials for the microparticles.
- **Option B**: "The technique uses a micro manipulator to inject DNA." This statement is false. The gene gun method does not use a micro manipulator; instead, it relies on the physical bombardment of DNA-coated particles.
- **Option C**: "It is also known as biolistics." This statement is true since the gene gun method is indeed referred to as the biolistic method.
- **Option D**: "Both 2 and 3." Since option 2 is false and option 3 is true, this option is also incorrect.

3. **Identifying the Incorrect Statement**: The question asks for the statement that is not true regarding the gene gun method. Based on the analysis, option B is the only statement that is not true.

4. **Conclusion**: Therefore, the answer to the question is option B, which states that the technique uses a micro manipulator to inject DNA.

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