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A suitable method of transformation with...

A suitable method of transformation with recombinant DNA for plants in which cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is:

A

Biolistics

B

Microinjection

C

Gene gun

D

Both (1) and (3)

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AI Generated Solution

The correct Answer is:
To solve the question regarding the suitable method of transformation with recombinant DNA for plants, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding the Question**: The question asks for a method of transformation in plants that involves bombarding cells with high-velocity micro-particles coated with DNA. 2. **Identifying Key Terms**: The key terms in the question are "high velocity micro-particles," "gold or tungsten," and "coated with DNA." These terms hint towards a specific technique used in plant biotechnology. 3. **Reviewing the Options**: - **Biolistic**: This method involves the use of a gene gun to shoot DNA-coated micro-particles into plant cells. - **Microinjection**: This method uses a fine needle to inject DNA directly into cells, which does not involve the use of micro-particles. - **Gene Gun**: This is another name for the biolistic method, where DNA-coated micro-particles are used to deliver genetic material into cells. - **Both first and third**: This option suggests that both the biolistic method and the gene gun are correct. 4. **Analyzing the Techniques**: - The biolistic method and gene gun are synonymous, as both refer to the technique of using high-velocity micro-particles to deliver DNA into plant cells. - Microinjection is not applicable here since it does not involve the use of micro-particles. 5. **Conclusion**: Since the biolistic method and gene gun are the same technique, the correct answer is that the suitable method of transformation is both the first and third options: **Biolistic and Gene Gun**. ### Final Answer: The suitable method of transformation with recombinant DNA for plants in which cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is: **Both first and third (Biolistic and Gene Gun)**. ---
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VMC MODULES ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-ENABLE
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  4. How many restriction endonuclease enzymes have been isolated till date...

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  9. The cells of E. coli may be made competent to take up external DNA in ...

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  10. Amongst the following statements which one is not valid w.r.t. restric...

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  12. Which of the following statements is incorrect?

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  13. Mark the odd one out w.r.t. the practical use of the technique of PCR ...

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  14. The technique of extraction of DNA fragments from the gel is called:

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  15. In cloning vector pBR322, the Barn HI cloning site is found in which s...

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  16. The linking of antibiotic resistance gene with the plasmid vector beca...

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  17. The technique used for separation of DNA fragment is:

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  18. The restriction site Pvu II is included in:

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  19. Vectors constructed by combining certain features of plasmid and the c...

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  20. In the PCR technique, the heat resistant polymerase used is :

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