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X-gal is added in a culture medium conta...

X-gal is added in a culture medium containing recombi- nant plasmid, which has inactivated sequence for `alpha`-ga- lactosidase enzyme due to insertion of a foreign DNA. Which of the following condition will occur in this case?

A

Colour will change

B

No colour will change

C

Only slight colour change will occur

D

Gene will become active

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To solve the question regarding the addition of X-gal in a culture medium containing a recombinant plasmid with an inactivated sequence for alpha-galactosidase, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding the Components**: - X-gal is a substrate that is used in molecular biology to detect the activity of the enzyme beta-galactosidase. - The recombinant plasmid mentioned has an inactivated sequence for the enzyme alpha-galactosidase due to the insertion of foreign DNA. 2. **Recognizing the Role of Beta-galactosidase**: - Beta-galactosidase is an enzyme that, when active, can hydrolyze X-gal to produce a blue-colored product. This is a crucial part of the blue-white selection method used in cloning. 3. **Insertional Inactivation**: - The insertion of foreign DNA into the plasmid disrupts the gene coding for beta-galactosidase, leading to its inactivation. This process is known as insertional inactivation. 4. **Expected Outcome with X-gal**: - Since the recombinant plasmid has an inactivated beta-galactosidase gene, when X-gal is added to the culture medium, there will be no enzyme to react with X-gal. Therefore, no blue color will be produced. 5. **Comparison with Non-Recombinant Plasmids**: - Non-recombinant plasmids, which have an active beta-galactosidase gene, will react with X-gal and produce a blue color. However, in this case, we are focusing on the recombinant plasmid. 6. **Conclusion**: - The condition that will occur when X-gal is added to the culture medium containing the recombinant plasmid is that **no color change will occur**. ### Final Answer: No color change will occur when X-gal is added to the culture medium containing the recombinant plasmid.
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