The restriction site Pvu II is included in:

Options

A. tet R gene
B. amp R gene
C. rop gene
D. ori gene

Answer

To determine where the restriction site Pvu II is included, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Question**: The question asks about the restriction site Pvu II and where it is found within a specific context, likely referring to a plasmid vector.

2. **Identify the Plasmid Vector**: The common plasmid vector mentioned in the context is PBR 322. This vector is often used in molecular biology for cloning purposes.

3. **Examine the Options**: The options provided are:
- Tetracycline resistance gene
- Ampicillin resistance gene
- ROP gene
- Ori gene

4. **Analyze Each Option**:
- **Tetracycline Resistance Gene**: This gene is associated with the restriction sites Bam HI and Sal I, so it does not include Pvu II.
- **Ampicillin Resistance Gene**: This gene has recognition sites for Pst I and Pvu I, which means it also does not include Pvu II.
- **ROP Gene**: The ROP gene is known to have the recognition site for Pvu II. This suggests that Pvu II is indeed included in this gene.
- **Ori Gene**: The origin of replication (Ori) typically does not contain recognition sites for restriction enzymes, as the presence of such sites could interfere with the replication process.

5. **Conclusion**: Based on the analysis, the correct answer is that the restriction site Pvu II is included in the ROP gene.

### Final Answer:
The restriction site Pvu II is included in the ROP gene.