Insect tolerant gene Bacillus thuringiensis is introduced using Ti-plasmid of :

Options

A. Escherichia coli
B. Arabidopsis thaliatla
C. Haemophilus influenzae
D. Agrobacterium tumefaciens

Answer

To answer the question regarding the introduction of the insect-tolerant gene from Bacillus thuringiensis using Ti-plasmid, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Source of the Insect Tolerant Gene**:
- The insect-tolerant gene comes from Bacillus thuringiensis (Bt), which is a bacterium known for its ability to produce proteins toxic to certain insects.

2. **Understand the Role of Ti-plasmid**:
- Ti-plasmid (Tumor-inducing plasmid) is a plasmid found in Agrobacterium tumefaciens, which is a bacterium that can transfer genetic material into plants.

3. **Recognize the Function of Agrobacterium**:
- Agrobacterium tumefaciens is commonly used in genetic engineering to introduce new genes into plant cells. The Ti-plasmid facilitates the transfer of the desired gene into the plant genome.

4. **Determine the Correct Option**:
- Among the provided options, the only organism that is known to utilize Ti-plasmid for gene transfer is Agrobacterium tumefaciens. The other options (Escherichia coli, Arabidopsis thaliana, and Haemophilus influenza) do not utilize Ti-plasmid for this purpose.

5. **Conclude the Answer**:
- Therefore, the insect-tolerant gene from Bacillus thuringiensis is introduced using the Ti-plasmid of **Agrobacterium tumefaciens**.

### Final Answer:
The insect-tolerant gene Bacillus thuringiensis is introduced using the Ti-plasmid of **Agrobacterium tumefaciens**.

---