Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

Options

A. The enzyme cuts DNA molecule at identified position within the DNA
B. The enzyme binds DNA at specific sites and cuts only one of the two strands
C. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
D. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA

Answer

To identify the incorrect statement regarding the characteristics of the enzyme Restriction Endonuclease, we will analyze each statement provided in the question step by step.

### Step-by-Step Solution:

1. **Understanding Restriction Endonucleases**:
- These are bacterial enzymes that function as molecular scissors. They cut DNA at specific sites, which are usually palindromic sequences.

2. **Analyzing the Statements**:
- **Statement 1**: "The enzyme cuts the DNA molecule at the identified position within the DNA."
- This statement is correct. Restriction endonucleases do cut DNA at specific identified positions.

- **Statement 2**: "The enzyme binds the DNA at specific sites and cuts only one of the two strands."
- This statement is incorrect. Restriction endonucleases cut both strands of the DNA double helix, not just one.

- **Statement 3**: "The enzyme cuts the sugar phosphate backbone at the sides of each strand."
- This statement is correct. The enzyme does cut the sugar-phosphate backbone of both strands at specific sites.

- **Statement 4**: "The enzyme recognizes a specific palindromic nucleotide sequence in DNA."
- This statement is also correct. Restriction endonucleases recognize specific palindromic sequences before cutting.

3. **Conclusion**:
- The incorrect statement among the provided options is **Statement 2**: "The enzyme binds the DNA at specific sites and cuts only one of the two strands."

### Final Answer:
The incorrect statement is **Statement 2**.

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