Humulin is:

Options

A. introducing sequence of human proinsulin in E.coli
B. introducing sequence of A and B chains of Human insulin in single E.coli
C. introduction sequence of A and B chain of human insulin in separate E.coli
D. introduction insulin gene in E.coli using PUC18 vector

Answer

**Step-by-Step Solution:**

1. **Understanding Humulin:**
- Humulin is a recombinant form of human insulin that was developed through genetic engineering. It was the first drug produced using this technology and received approval from the FDA (Food and Drug Administration).

2. **Genetic Engineering Process:**
- The process involves inserting the human genes responsible for insulin production into a bacterial host, specifically *E. coli*. This allows the bacteria to produce insulin that is biologically identical to human insulin.

3. **Analyzing the Statements:**
- We have four statements regarding the production of Humulin:
1. Introducing the sequence of human pro-insulin in *E. coli*.
2. Introducing the sequence of A and B chains of human insulin in a single *E. coli*.
3. Introducing the sequence of A and B chains of human insulin in separate *E. coli*.
4. Introducing the insulin gene in *E. coli* using the PUC18 vector.

4. **Evaluating Each Statement:**
- **Statement 1:** Incorrect. Pro-insulin is the precursor to insulin, not the final product used in Humulin.
- **Statement 2:** Incorrect. The A and B chains of insulin are introduced in separate *E. coli* cells, not in a single one.
- **Statement 3:** Correct. The production of Humulin involves introducing the A and B chains of human insulin into separate *E. coli*.
- **Statement 4:** Incorrect. While a vector is used in the process, the specific mention of PUC18 is not accurate for the production of Humulin.

5. **Conclusion:**
- The correct statement regarding Humulin is: "Introduction of the sequence of A and B chains of human insulin in separate *E. coli*."