The importance of dicer enzyme in RNA interference technique is that it:

Options

A. causes degrasation of nematode m- RNA
B. silencing of RISC
C. cleaves dsRNA to siRNA
D. reduction in surface area of roots of host plants

Answer

**Step-by-Step Solution:**

1. **Understanding RNA Interference (RNAi):**
- RNA interference is a biological process where RNA molecules inhibit gene expression or translation, effectively silencing specific genes. This is a natural defense mechanism found in eukaryotic cells.

2. **Role of Dicer Enzyme:**
- The Dicer enzyme is crucial in the RNA interference pathway. It processes long double-stranded RNA (dsRNA) into shorter fragments known as small interfering RNA (siRNA).

3. **Formation of siRNA:**
- Dicer cleaves the dsRNA into approximately 20-25 nucleotide long siRNA molecules. This step is essential because siRNA is the active form that will participate in the silencing of target mRNA.

4. **Interaction with RISC:**
- Once formed, siRNA is incorporated into the RNA-induced silencing complex (RISC). The siRNA guides RISC to the complementary mRNA target.

5. **Cleavage of Target mRNA:**
- The RISC, with the help of siRNA, binds to the target mRNA and cleaves it, leading to the degradation of the mRNA. This prevents the mRNA from being translated into protein, effectively silencing the gene.

6. **Conclusion:**
- Therefore, the importance of the Dicer enzyme in the RNA interference technique is that it cleaves dsRNA into siRNA, which is essential for the subsequent silencing of target mRNA.

**Final Answer:**
The importance of the Dicer enzyme in RNA interference technique is that it cleaves dsRNA into siRNA.

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