Hybridomas are the fusion product of :

Options

A. normal antibody producing cell with myeloma
B. abnormal antibody producing cell with myeloma
C. sex cells with myeloma
D. bone cell with myeloma

Answer

**Step-by-Step Solution:**

1. **Understanding Hybridoma**: The term "hybridoma" refers to a type of cell that is created by the fusion of two different types of cells. The prefix "hybrid" indicates a combination of two distinct entities.

2. **Function of Hybridomas**: Hybridomas are primarily used in biotechnology for the production of monoclonal antibodies. Monoclonal antibodies are identical antibodies produced by a single clone of cells and are specific to a particular antigen.

3. **The Fusion Process**: The process of creating hybridomas involves fusing myeloma cells with normal antibody-producing cells. Myeloma cells are a type of cancerous B cell that can divide indefinitely and produce antibodies.

4. **Source of Normal Antibody-Producing Cells**: The normal antibody-producing cells are typically derived from the spleen of a mouse that has been immunized with a specific antigen. This immunization prompts the mouse's immune system to produce antibodies against that antigen.

5. **The Resulting Hybridoma**: When the myeloma cells are fused with the spleen cells, the resulting hybridoma cells possess the ability to proliferate indefinitely (due to the myeloma component) and produce specific antibodies (due to the spleen cell component).

6. **Culturing Hybridomas**: After fusion, the hybridomas are cultured in a selective medium, often referred to as HAT medium, which allows only the hybridoma cells to survive and grow.

7. **Harvesting Monoclonal Antibodies**: Once the hybridomas are established, they can be screened for the production of the desired monoclonal antibodies, which can then be harvested for various applications in research, diagnostics, and therapeutics.

**Final Answer**: Hybridomas are the fusion product of normal antibody-producing cells (from the spleen) and myeloma cells.

---