Which is correct regarding genetically engineered insulin using E.coli ?

Options

A. diffcult to purity
B. obtained in larger unlimited quantities
C. possibility of transmission of animal diseases
D. insulin obtained varies in chemical structure

Answer

**Step-by-Step Solution:**

1. **Understanding the Context**: The question asks about genetically engineered insulin produced using E. coli. This involves the use of recombinant DNA technology to produce human insulin.

2. **Production Process**: Human insulin is produced by isolating the DNA sequences that code for the A and B chains of insulin. These sequences are then inserted into E. coli bacteria, which serve as a vector.

3. **Bacterial Growth**: The E. coli bacteria are cultured, allowing them to multiply. As they grow, they express the insulin chains.

4. **Isolation of Insulin Chains**: After a certain period, the bacteria are lysed (broken open) to release the insulin chains (A and B).

5. **Joining the Chains**: The A and B chains are then chemically linked together using disulfide bonds to form functional human insulin.

6. **Evaluating the Options**:
- **Option 1**: "Difficult to purify" - This is incorrect because downstream processing is employed to improve the purity of insulin.
- **Option 2**: "Obtained in large quantities" - This is correct as E. coli can multiply rapidly, allowing for the production of large amounts of insulin.
- **Option 3**: "Possibility of transmission of animal disease" - This is incorrect because the production occurs in controlled conditions, minimizing contamination risks.
- **Option 4**: "Insulin obtained varies in chemical nature" - This is incorrect as the insulin produced is identical to human insulin in structure.

7. **Conclusion**: The correct statement regarding genetically engineered insulin using E. coli is that it is "obtained in large quantities."

**Final Answer**: The correct option is that insulin is "obtained in large quantities."

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