RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in preventing the process of:

Options

A. transcription
B. translation of mRNA
C. replication of DNA
D. splicing of hnRNA

Answer

To solve the question regarding RNA interference (RNAi) and its role in making tobacco plants resistant to Meloidogyne incognita, we can break down the process into the following steps:

### Step-by-Step Solution:

1. **Understanding RNA Interference (RNAi)**:
- RNA interference is a biological process where RNA molecules inhibit gene expression or translation by neutralizing targeted mRNA molecules.

2. **Role of RNAi in Plant Resistance**:
- In the context of tobacco plants, RNAi is utilized to combat the nematode Meloidogyne incognita. This nematode can cause significant damage to the plants.

3. **Mechanism of RNAi**:
- When the nematode infects the tobacco plant, RNAi technology is employed to silence specific genes that are crucial for the nematode's survival and reproduction.
- A complementary RNA strand is synthesized that matches the RNA of the nematode. When these complementary strands bind together, they form double-stranded RNA (dsRNA).

4. **Impact of Double-Stranded RNA**:
- The formation of dsRNA prevents the translation of mRNA into proteins. Since proteins are essential for the nematode's life cycle and pathogenicity, blocking their production helps in controlling the infestation.

5. **Conclusion**:
- Therefore, the process that RNA interference is essentially involved in preventing is the **translation of mRNA**.

### Final Answer:
The correct answer is **translation of mRNA**.

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