Which one of the following crosses will produce two phenotypes in next generation?
A. Out cross
B. Selfing of pure lines
C. Test cross
D. Incomplete dominance

Options

A. A. Out cross
B. B. Selfing of pure lines
C. C. Test cross
D. D. Incomplete dominance

Answer

To determine which of the given crosses will produce two phenotypes in the next generation, let's analyze each option step by step.

### Step-by-Step Solution:

1. **Understanding the Question**: We need to identify which cross results in two distinct phenotypes in the offspring.

2. **Option A: Out Cross**:
- An outcross involves crossing two different individuals. The results can be unpredictable, and it does not guarantee two distinct phenotypes. Therefore, this option is not correct.

3. **Option B: Selfing of Pure Lines**:
- Selfing of pure lines means breeding individuals that are homozygous for a trait. This will produce offspring that are all uniform (homozygous) for that trait, resulting in only one phenotype. Hence, this option is also not correct.

4. **Option C: Test Cross**:
- A test cross involves crossing an individual with a dominant phenotype (but unknown genotype) with a homozygous recessive individual. This can produce two different phenotypes in the offspring: one showing the dominant trait and the other showing the recessive trait, depending on whether the dominant individual is homozygous or heterozygous. Therefore, this option is correct.

5. **Option D: Incomplete Dominance**:
- Incomplete dominance occurs when two alleles blend to produce a third phenotype, which is a mix of the two. This does not produce two distinct phenotypes but rather a new one. Therefore, this option is not correct.

### Conclusion:
The correct answer is **Option C: Test Cross**, as it can produce two distinct phenotypes in the next generation.

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