The correct sequence in the tissue culture technique is

Options

A. Inoculation explant incubation callus formation organogenesis preparation of medium transferred to field
B. Preparetion of medium inoculation explant incubation callus formation organogenesis transferred to field
C. Explant incubation preparation of medium inoculation organogenesis callus formation-transferred to field.
D. Preparation of medium inoculation explant incubation organogenesis callus formation transferred to field.

Answer

To determine the correct sequence in the tissue culture technique, we can break down the process into its essential steps. Here’s a step-by-step solution:

### Step-by-Step Solution:

1. **Preparation of the Medium**: The first step in tissue culture is to prepare the growth medium. This medium can be in the form of broth or agar and must contain the appropriate nutrients necessary for plant growth.

2. **Inoculation**: After preparing the medium, the next step is to inoculate it with the selected plant tissue (explant). This involves placing the explant into the prepared medium.

3. **Explant Incubation**: Once the explant is inoculated, it is incubated under specific conditions. This includes maintaining the right temperature and environmental conditions to promote growth.

4. **Callus Formation**: During the incubation, the unorganized tissue mass begins to grow, forming what is known as callus. This is an essential phase where the tissue starts to proliferate.

5. **Organogenesis**: Following callus formation, the next step is organogenesis, where the callus differentiates into various plant organs (roots, shoots, etc.).

6. **Transfer to the Field**: The final step involves transferring the developed plantlets to the field for further growth and development.

### Correct Sequence:
- Preparation of the medium
- Inoculation
- Explant incubation
- Callus formation
- Organogenesis
- Transfer to the field

### Final Answer:
The correct sequence in the tissue culture technique is: **Preparation of the medium, Inoculation, Explant incubation, Callus formation, Organogenesis, Transfer to the field.**

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