(i) Explain, how to find whether an E.coli bacterium has transformed or not, when a recombinant DNA bearing ampicillin-resistant gene is transferred into it. (ii) What does the ampicllin-resistant gene act as, in the above case ?

Using recombinant DNA technology, genes from a donor cell can be inserted into a bacterium for DNA replication and protein synthesis. The kind of cells that can be used as gene donors in this technology are

How recombinant DNA technology is useful is detecting the presence of mutated genes?

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Read the given statements and select the correct option. Statement 1 : The tumour inducing plasmid (Tiplasmid) acts as a cloning vector in recombinant DNA technology. Statement 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be (a) Human gene may have intron which bacteria cannot process (b) Amino acid codons for humans and bacteria are different (c) Human protein is formed but degraded by bacteria (d) all of the above

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.